FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617616
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC8940.14475013600683923Illumina Single End PCR Primer 1 (97% over 35bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC7170.11609155203233076No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA7030.11382477137897981No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA6840.11074842620657496No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG6580.10653869070749461No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC6490.1050814745731976No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT6380.10330043263127899No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA6350.10281469391984664No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG6190.10022408745887412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT1800.060.26962337
CGGTGGT2150.060.22440752
GGTGGTC2300.054.77519653
ATAGTGT1800.054.4370839
GCTATAG1950.053.84322736
TCGGTGG2150.053.7136651
GGTCGCC2550.050.77744356
TAGTGTA1950.050.2496140
AGAGTGT3000.049.0132127
CCCTATC506.2018444E-848.9933761
GTCGCCC604.940375E-946.6603557
CGCCCTA604.940375E-946.6603559
GTGGTCG2800.046.24374454
CGTGTAG3800.046.0538616
GTAGGGA3650.046.0286219
GTCGCCG2650.044.89958657
TGTAGGC3450.044.646131
GGCTATA3300.043.48600435
GAGTGTA3450.042.61673428
TCGTGTA4700.042.4479415