Basic Statistics
Measure | Value |
---|---|
Filename | HLNTYAFXY_n02_PY79_T3_R1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 617616 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC | 894 | 0.14475013600683923 | Illumina Single End PCR Primer 1 (97% over 35bp) |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 717 | 0.11609155203233076 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 703 | 0.11382477137897981 | No Hit |
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA | 684 | 0.11074842620657496 | No Hit |
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG | 658 | 0.10653869070749461 | No Hit |
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC | 649 | 0.1050814745731976 | No Hit |
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT | 638 | 0.10330043263127899 | No Hit |
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 635 | 0.10281469391984664 | No Hit |
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG | 619 | 0.10022408745887412 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATAGT | 180 | 0.0 | 60.269623 | 37 |
CGGTGGT | 215 | 0.0 | 60.224407 | 52 |
GGTGGTC | 230 | 0.0 | 54.775196 | 53 |
ATAGTGT | 180 | 0.0 | 54.43708 | 39 |
GCTATAG | 195 | 0.0 | 53.843227 | 36 |
TCGGTGG | 215 | 0.0 | 53.71366 | 51 |
GGTCGCC | 255 | 0.0 | 50.777443 | 56 |
TAGTGTA | 195 | 0.0 | 50.24961 | 40 |
AGAGTGT | 300 | 0.0 | 49.01321 | 27 |
CCCTATC | 50 | 6.2018444E-8 | 48.99337 | 61 |
GTCGCCC | 60 | 4.940375E-9 | 46.66035 | 57 |
CGCCCTA | 60 | 4.940375E-9 | 46.66035 | 59 |
GTGGTCG | 280 | 0.0 | 46.243744 | 54 |
CGTGTAG | 380 | 0.0 | 46.05386 | 16 |
GTAGGGA | 365 | 0.0 | 46.02862 | 19 |
GTCGCCG | 265 | 0.0 | 44.899586 | 57 |
TGTAGGC | 345 | 0.0 | 44.6461 | 31 |
GGCTATA | 330 | 0.0 | 43.486004 | 35 |
GAGTGTA | 345 | 0.0 | 42.616734 | 28 |
TCGTGTA | 470 | 0.0 | 42.44794 | 15 |