FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T2_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T2_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835334
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC12950.1550278092355872Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG2350.061.0733951
CGGTGGT2550.056.28331852
ATAGGGT2100.054.99543837
GATAGGG2300.050.2102236
GGTGGTC3000.049.0076753
CGCCCTA651.8553692E-1048.46912459
GGTCGCC3000.046.67117756
GTAGGAT3350.045.96358532
TAGGGTG2400.045.20458238
CCCTATC703.8380676E-1045.00704661
TAGGATA3400.043.2291233
GGGTGTA2600.043.0733540
GTAGGGA3550.042.39338719
GGTGTAG3450.041.59075541
ACTACCC855.638867E-1141.1730546
TACTACC855.638867E-1141.1730545
GTGGTCG3700.040.68204554
GTCGTGT5050.040.1970914
AGAGTGT3150.039.9966827
TAGTGTA701.9286745E-839.9966828
Produced by FastQC (version 0.11.5)