FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T2_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T2_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829646
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC10430.12571626934861374Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC200.006953772652.49538835
TCGGTGG1700.051.4691751
CGGTGGT1850.049.1878352
GGTGGTC2150.045.5801453
GTGTAGA2000.043.74879542
GGTCGCC2500.040.5988856
CGTGTAG2650.039.62154816
GCCTCTA2550.037.0555734
GTAGGGA2850.036.8410919
GTGGTCG2900.036.205954
AGTATAG400.00241937334.9990446
AGAGTGT2600.033.6529227
AGTGTGC2700.032.40651329
GTCGTGT3350.032.3871714
GTAGATC2850.031.92894444
ATCTCGG3000.031.49913248
TAGATCT2800.031.2491445
ACTAGCG450.00430835931.1102523
TCTATGT3050.030.98462137
AGATCTC2950.030.84660746
Produced by FastQC (version 0.11.5)