FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995390
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT1900.049.73930452
TCGGTGG1850.047.2996451
GTAGGGA2200.044.5454219
GGTCGCC1900.044.21049556
GGTGGTC2000.043.75436453
CTATAGT1150.042.60010537
TACTACC503.4816348E-641.997865
GCTATAG1200.040.82304836
CTCCTAG459.198327E-538.89081651
CACTAGT459.214675E-538.8790936
TAGTGTA1250.036.3926640
ACTAGTA601.2180644E-534.99294337
GTCGTGT3650.034.52052314
TGTAGGC2250.034.222231
AGAGTGT2300.033.47823727
CGTGTAG3050.033.27866416
CACCCTA552.9914183E-431.82135666
TGTAGGG3450.030.4347618
GTGGTCG2850.029.47662554
AGCGTCG4600.028.91157211
Produced by FastQC (version 0.11.5)