FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_6_ctrl.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_6_ctrl.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35910299
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA2691850.7496038949717461No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA1370680.38169551303374No Hit
CTCAACTTTACCGGATTTCAAAGCTCGAGCAGATACATATACTTTTTTAG1017520.2833504672294708No Hit
ATTTTTTGTTAAAGCGATCAACACGACCATCAGCAGAAGCGAATTTTTGA959550.2672074660252759No Hit
GAAGATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGA942680.26250964939055504No Hit
TTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAG933350.25991150895179127No Hit
TGAAGATTGACTTTTCGTCTTGATGATACTTTGGGCAAGGATAGTATATA829300.23093653439087208No Hit
CTAACGTTCTGTAAGCCATGTTTTGTTCCATCGTACTGCAAACGGCTCAT811530.2259880932765277No Hit
CGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAAA759730.211563262115974No Hit
ATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGAAACA633270.17634773801242926No Hit
GTCCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCT613570.17086184662511444No Hit
CTCGATTACAATACCCATACTGCGGGCAGTACCTTCAACCATGCGCATTG599720.1670050143553525No Hit
CTCCGAAATAATAACTCTGTTTCCACACGGGATTCAGGGCGGAAGGAGAT580110.16154418541600002No Hit
CCGGCAACTGCGTCTTTAAGCGCTTTACCTGGTTTGAAAGCAGGTACTTT517620.14414249238080698No Hit
GTCCGTTCACTTTTTTCGCTAGTCTTGATTTGTATCGAGCTGCGGTATTT515350.14351036174886764No Hit
CTTGAATCTGTAGAGGAAAGTCCATGCTCGCACGGTGCTGAGATGCCCGT487280.1356936627010541No Hit
GTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAGCAAGAATAGTGA455940.12696636137727507No Hit
CTAGTTTGTTAATTAACTCACGGTAACGAGTTACGTCTTTATTACGCAGA448720.1249557961074064No Hit
TGCAGATCTTGAATCTGTAGAGGAAAGTCCATGCTCGCACGGTGCTGAGA445760.12413152004108906No Hit
CGCTTCTTTAGTAACGTTAGCAGCTTGTGGTCCGCGGTTTCCTTCAACGA426080.11865119808665477No Hit
CGAAATAATAACTCTGTTTCCACACGGGATTCAGGGCGGAAGGAGATGGA398570.11099044315949583No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG393780.10965656398461066No Hit
CTATCTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTT359910.10022472940144553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT201150.032.00251
GGTGGCA241350.030.92059367
GGGTGGC246350.030.17943266
GTCTAAC61100.028.374121
CTCCGAA163400.027.8215331
AGATTTA265800.027.6438963
AGAAAGT748650.027.5422429
TGGGTGG272400.027.4488765
TAAGAGT756150.027.13157520
AGTGTAG286800.026.89839756
CTCAACT316350.026.803121
GTGGCAT283150.026.52827368
TGTAAGA786700.026.25198218
CTATCTT104750.026.246661
TACTGCG177100.025.72989718
TTGTAAG802300.025.7197617
CCATACT177250.025.68826915
GAGGCAA247900.025.65516570
ACGAACC307400.025.585670
ACACGAC304800.025.35369321
Produced by FastQC (version 0.11.5)