FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_6_Fe500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_6_Fe500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19721193
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA302470.15337307433683145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT84950.039.2046171
AGGCCTA92600.035.56729522
CGATCGA53750.033.602114
TCGATCG54650.032.538253
CGATTAG54000.032.4043468
GGCCTAA102600.032.20238523
CAGGCCT104450.031.49838421
TTAGTAT56450.031.06003611
GCCTAAC112450.029.5062824
CAGATTG116600.028.9400423
TCAGATT117950.028.7602562
CGGCAGG115900.028.71776218
CTCGATC62600.028.6327152
GTCGAAC87000.028.35752139
TGAACGC118850.028.2682638
AGATTGA133250.027.5026654
GATCGAT67800.026.9969355
GGCGGCA135500.024.74455816
ACACATG134400.024.55789829
TTGAACG147950.023.7727417
Produced by FastQC (version 0.11.5)