FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_5_ctrl.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_5_ctrl.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26812181
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA1858390.6931140737860899No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA801260.29884178388919574No Hit
CTCAACTTTACCGGATTTCAAAGCTCGAGCAGATACATATACTTTTTTAG737490.2750578179373025No Hit
ATTTTTTGTTAAAGCGATCAACACGACCATCAGCAGAAGCGAATTTTTGA643040.23983129160585628No Hit
CTAACGTTCTGTAAGCCATGTTTTGTTCCATCGTACTGCAAACGGCTCAT564180.2104192866667579No Hit
TGAAGATTGACTTTTCGTCTTGATGATACTTTGGGCAAGGATAGTATATA555040.20701038830075033No Hit
TTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAG535750.19981589711034697No Hit
GAAGATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGA508280.18957055377180992No Hit
CTCCGAAATAATAACTCTGTTTCCACACGGGATTCAGGGCGGAAGGAGAT468740.17482352517312932No Hit
CGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAAA444160.16565605013631676No Hit
CTCGATTACAATACCCATACTGCGGGCAGTACCTTCAACCATGCGCATTG394640.14718683273098895No Hit
GTCCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCT386100.14400171325115252No Hit
CTAGTTTGTTAATTAACTCACGGTAACGAGTTACGTCTTTATTACGCAGA384460.14339005096228463No Hit
CCGGCAACTGCGTCTTTAAGCACTTTACCTGGTTTGAAAGCAGGTACTTT366850.1368221406531606No Hit
ATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGAAACA364230.1358449728502131No Hit
CTTGAATCTGTAGAGGAAAGTCCATGCTCGCACGGTGCTGAGATGCCCGT325460.12138512715545222No Hit
GTCCGTTCACTTTTTTCGCTAGTCTTGATTTGTATCGAGCTGCGGTATTT308930.11522001884143628No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300290.11199760288057134No Hit
CTATCTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTT285810.10659707242764026No Hit
CGAAATAATAACTCTGTTTCCACACGGGATTCAGGGCGGAAGGAGATGGA270190.10077136209098395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAA121950.031.8488181
GGTGGCA159000.031.76699667
CTAACGT146250.030.6040251
GGGTGGC166900.030.36826766
TAAGAGT491300.028.230420
ACGAACC205000.027.86518370
TGGGTGG184750.027.5111965
CTATCTT84600.027.4878831
CTCAACT230850.027.1864011
GTGGCAT190100.026.77162268
TGTAAGA521500.026.76365318
AGTGTAG192750.026.71241256
GAGGCAA156950.026.20427570
TTGTAAG534850.026.16111217
GGAGGCA158650.025.81302669
AGAAAGT541100.025.8117359
CACTAGG160350.025.56309964
TACCGGA237150.025.5438849
ACCGGAT236200.025.51327710
GGCATTC197350.025.34490670
Produced by FastQC (version 0.11.5)