FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_5_Fe500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_5_Fe500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18969958
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA206040.10861384089516699No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198200.10448099041653124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGTG75600.021.57515356
GAATAGT75450.021.52554955
GTAAGAG91800.017.91830319
TAAGAGT94600.017.53588720
CTAACGT61100.017.13761
GGGTGGC22050.016.9852966
TAAGCCA60350.016.7010712
GGTGGCA23000.016.2836867
GCCATGT63150.015.57296315
GTAAGCC66100.015.30103811
AGAATAG108600.015.18039854
TACTCAG75100.014.49515458
ACACTAG25700.014.43743363
TACTGCG38600.014.14425718
TGTGCTA22900.014.06131922
TAGTGAA118200.014.00702958
GGGTACC9300.013.92278535
AGTGAAT122400.013.66988659
TCTAGAC11300.013.62871250
ACCTCGT70350.013.48297752
Produced by FastQC (version 0.11.5)