FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_4_ctrl.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_4_ctrl.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25963222
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA1171390.45117281668661924No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA529900.2040963945075846No Hit
CTCAACTTTACCGGATTTCAAAGCTCGAGCAGATACATATACTTTTTTAG423490.16311149671639366No Hit
TTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAG405620.15622868378970836No Hit
ATTTTTTGTTAAAGCGATCAACACGACCATCAGCAGAAGCGAATTTTTGA380470.14654190454482113No Hit
TGAAGATTGACTTTTCGTCTTGATGATACTTTGGGCAAGGATAGTATATA361490.13923156378665175No Hit
GAAGATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGA326530.12576636289594567No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG317170.1221612633439717No Hit
CGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAAA292000.11246678089491358No Hit
CTAACGTTCTGTAAGCCATGTTTTGTTCCATCGTACTGCAAACGGCTCAT289510.11150773197563847No Hit
GTCCGTTCACTTTTTTCGCTAGTCTTGATTTGTATCGAGCTGCGGTATTT264640.10192879758914361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTA38700.036.53529733
ATAGTGT35900.034.5085639
TCGGTGG37250.034.1090451
GTAGGGA51500.030.64902319
TGGTCGC44750.030.270555
TATAGTG39750.030.0213538
GGTGGCA100950.029.61095267
GTGGTCG46600.029.44428454
GGGTGGC106400.028.12712366
CTATAGT44850.028.0126537
GCTATAG45950.027.34057836
TAGTGTA46850.026.7421140
GTAGGCT52650.026.52332
CTCGGTG41500.026.06135650
TAAGAGT357200.025.278620
CTAACGT97700.024.5553551
AGGCTAT59650.023.93665934
TGGGTGG126150.023.86329765
GAATAGT376400.023.7598155
AATAGTG377700.023.6494156