FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_4_Fe500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_4_Fe500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25184638
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA387400.15382393028639127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262280.104142850891881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGCA48900.021.8321167
GGGTGGC50100.021.44904366
AATAGTG136400.020.47761756
GAATAGT136850.020.46224855
ACACTAG46450.019.21655563
TGGGTGG57900.018.86251865
TAAGAGT154000.018.58970820
TGTGCTA45550.018.36386922
GTAAGAG158150.017.94706219
AGTGTAG64450.017.16149356
ACACGAC68250.016.56392121
CTAACGT80800.016.3852961
AGAATAG177150.016.06401454
CACTAGG57150.015.61870564
CACACTA58650.015.21931162
CTCAACT85350.015.0193611
TAGTGAA193100.014.73724858
GTGTGGT59750.014.64555753
ACTCTAG21450.014.3577335
GCCATGT86250.014.28333415
Produced by FastQC (version 0.11.5)