FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17573636
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA194830.11086493426858278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAGT72350.023.38195620
CTAACGT28300.023.035421
GGGTGGC21400.021.75324866
AATAGTG73000.021.28237556
TGTAAGA81700.021.07138818
TGTGCTA24750.020.9456922
TTGTAAG84800.020.77735317
CACGTCT39850.020.43597415
GAACTCC37350.020.35060322
AGATCGG41400.020.1486631
AGAAAGT90600.019.5526399
ACACGTC41850.019.4587914
CTCAACT34300.019.00591
ACACTAG23650.018.9484163
AGTGTAG24850.018.44611556
TGTTAAG88150.018.34455143
AGAATAG87350.018.31711654
AAGAGTT95400.018.13752621
AGTGAAT86300.017.80552559
TAGTGAA87150.017.7918158
Produced by FastQC (version 0.11.5)