FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surE_T4_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surE_T4_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001575
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA124451.2425429947832165TruSeq Adapter, Index 7 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA29320.29273893617552355No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT28780.2873474278012131TruSeq Adapter, Index 7 (97% over 36bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC28570.2852507301000924No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT25850.2580935027331952No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA25840.2579936599855228No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG25090.2505054539100916No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA19390.19359508773681453No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA14960.14936475051793424No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC14880.1485660085365549No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT14270.14247560092853756No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA13300.1327908544043132No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT12840.12819808801138205No Hit
CCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTTCCTGACA12770.12749918877767516No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG12740.12719966053465792No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA12720.1269999750393131No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT12560.12540249107655443No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA12470.12450390634750268No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG12380.12360532161845093No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC12280.12260689414172678No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG12150.12130893842198537No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA11900.11881286973017496No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT11870.11851334148715773No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA11550.1153183735616404No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA11510.11491900257095075No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA11140.11122482090707136No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG10870.10852906671991613No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG10830.10812969572922645No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATGTCGTA10350.1033372438409505TruSeq Adapter, Index 7 (97% over 36bp)
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC10250.10233881636422634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG22200.051.3961446
GTATGCC22750.050.9228248
CGTATGC23000.050.67365647
GGCTATG24050.048.60682736
CTCGTAT20250.048.3948245
CCGGCTA24250.048.20594434
CTATGAT22450.047.8616838
TCACCGG24800.047.84250331
GCCGTCT23900.047.7403552
TATGCCG24950.047.13403349
CCGTCTT23850.047.1066853
AGTCACC25000.047.03976429
CGGCTAT24850.046.90117335
GCTATGA24550.046.6189137
CTCCAGT25650.046.1206325
CAGTCAC26150.045.90799328
ATGCCGT25500.045.568450
CCAGTCA26100.045.32544327
CACCGGC26100.045.19134532
ACTCCAG26400.045.0755324
Produced by FastQC (version 0.11.5)