FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surE_T4_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surE_T4_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872565
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA39650.4544074080440999TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT29940.34312630004641487TruSeq Adapter, Index 7 (97% over 36bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC22380.2564851902150556No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT18540.2124770074435715No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA17830.20434007781655236No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA17680.20262100817704126No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA17220.1973491946158739No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT15210.1743136614464252No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA14920.17099012681003706No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA14760.1691564525278919No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC13750.1575813836218505No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG13230.15162194220487873No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC12390.14199515222361658No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA12090.13855701294459438No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG11690.13397282723923146No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA11000.1260651068974804No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG10840.12423143261533524No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT10640.12193933976265378No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA10410.1193034329820701No Hit
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCT10310.11815738655572938No Hit
CCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTTCCTGACA9680.11093729406978278No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG9610.11013506157134426No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG9130.10463403872490874No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG8820.10108129480325247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTC200.00695048852.50189246
TACTACC1102.3646862E-1135.0032655
ACTACCC1055.493348E-1033.3364456
AGTCACC10800.033.0548529
TCACCGG10700.033.03668231
CCGGCTA10750.032.23187334
GGCTATG11100.031.84616936
AGATCGG11050.031.3639871
TCGTATG11000.030.86474646
CTATGAT10750.030.60438
GCCGTCT10850.030.32367352
GTATGCC11700.030.21476448
GAACTCC11950.029.8738422
CGTATGC11600.029.87176547
CACACGT12200.029.83543213
CAGTCAC12050.029.62592528
CGGCTAT11850.029.53523435
CCAGTCA12200.029.26167327
ACCGGCT11850.029.23988233
GCACACG12350.029.18966312
Produced by FastQC (version 0.11.5)