FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surE_T4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surE_T4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032700
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA30680.29708531035150576TruSeq Adapter, Index 7 (97% over 36bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC27520.2664859107194732No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC24720.23937251864045703No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG19590.18969691100997388No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA19510.1889222426648591No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA19180.18572673574126078No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT19140.18533940156870338No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA18420.17836738646267067No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT17480.16926503340757237No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA16510.15987217972305606No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG16090.15580517091120363No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA15370.1488331558051709No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG14940.14466931345017914No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT14520.1406023046383267No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG13960.13517962622252347No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT13700.13266195410090056No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG13610.13179045221264646No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT13260.12840127820276942No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA12690.12288176624382686No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG12620.12220393144185145No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA12600.12201026435557276No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG12320.11929892514767117No Hit
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCT10930.10583906265130241No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC10690.10351505761595817No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA10650.10312772344340079No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC10630.1029340563571221No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC10510.10177205383945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC7750.039.29129
TCGTATG7850.038.34646646
CACACGT8300.037.94878813
CTCTAGT2700.037.5914236
CAGTCAC8300.037.5307728
ACTCCAG8300.036.6873824
CTCCAGT8400.036.667325
CGTATGC8300.035.8457347
GTATGCC8400.035.8356948
CTATGAT8350.035.62590838
GGCTATG8500.035.40894736
CCAGTCA8950.035.1961427
TCACCGG8600.035.00060731
ACGTCTG9100.034.99721516
CACGTCT9050.034.8038615
CGGCTAT8650.034.7949235
TGTACTC3000.033.8322832
CTCGTAT7450.033.82608845
TCCAGTC9300.033.49520526
TACTACC1154.0017767E-1133.4772225
Produced by FastQC (version 0.11.5)