FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surE_T3_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surE_T3_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130947
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC19920.17613557487662995No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG18600.16446394039685325No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC17680.15632916485034223No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT17470.15447231391037775TruSeq Adapter, Index 7 (97% over 35bp)
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA17120.1513775623437703No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA16110.14244699353727452TruSeq Adapter, Index 7 (97% over 35bp)
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG14720.13015640874417633No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT14030.12405532708429307No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA13730.12140268288434383No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA13010.11503633680446564No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT11930.10548681768464835No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA11750.10389523116467882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC1004.4169974E-728.0054661
GTGATAG4500.028.001754
GTTACTA906.172444E-627.2239253
AGTCACG6700.026.12103728
CGTGATA5000.025.9016193
CGGGGAG2100.025.004881
TATCTCG5650.024.15866941
TAGCCTC5250.023.9983188
CACACGT7600.023.9456913
TGATAGC5600.023.7514845
CGTATCT6350.023.70017439
GGCGTGA4750.023.583551
ACTCCAG7600.023.02775624
ATTCGTA7800.022.43630837
TACTACC1253.0902775E-622.40145
AGTGGGG1253.093839E-622.3984311
CTAACGT2207.2759576E-1222.2770751
GTCACGA8350.022.21695929
AGTCGTA952.7826877E-422.10371466
GCACACG8150.021.90030511
Produced by FastQC (version 0.11.5)