FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surE_T3_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surE_T3_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901905
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA42320.4692290207948731TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT18300.20290385351007037TruSeq Adapter, Index 7 (97% over 35bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC17980.1993558079842112No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC13920.15433998037487318No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT12990.14402847306534503No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA12990.14402847306534503No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA12780.14170006818899994No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA12690.14070218038485205No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT12450.13804114624045769No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT12030.13338433648776754No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG11440.12684262754946474No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA11300.12529035763190136No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA10630.11786163731213375No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA10360.11486797389969011No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG10200.11309395113676052No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT10070.11165255764188024No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG9690.10743925357992251No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT9640.106884871466507No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA9630.1067739950438239No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG9410.10433471374479573No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA9100.10089754464161968No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG9080.10067579179625348No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA9040.1002322861055211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGG150.00222475669.9999553
AGTCACG10350.038.55069429
CAGTCAC11400.034.99997328
GTCACGA11500.034.69562530
TATCTCG8450.034.37867442
CCAGTCA11650.034.24889827
ATTCGTA11700.033.80339437
ACTCCAG12100.033.55369624
GTATGCC7950.033.4590948
GTATCTC9050.032.48616441
CGTATCT9850.032.33540
TCCAGTC12950.031.62159726
TTCGTAT12200.031.55735238
TCGTATC11350.031.4537239
GAATTCG12750.031.29409235
ACCTAGT450.00430799131.1110886
ACGAATT12700.030.8661233
CTAACGT1251.05501385E-1030.8016831
CTCCAGT13100.030.72516425
CGAATTC13300.030.26313434
Produced by FastQC (version 0.11.5)