FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surE_T2_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surE_T2_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682628
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA10210.14956901855769172TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT9980.14619968709165168TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG305.877959E-446.6662759
TACTACC801.4224497E-939.3775565
ACTACCC852.57387E-937.061236
AACCCTA400.002418846134.99970630
CTAGTAG1001.2589226E-831.49973538
CACTAGT1001.2589226E-831.49973536
GTATGCC2750.030.545247
GCCACTA1052.0241714E-829.99974834
TTACTAC952.8201612E-729.4755954
GTTACTA952.8201612E-729.4755953
ACTAGTA1103.1817763E-828.63612437
CTACCCT1103.1817763E-828.6361247
GGTGGTG755.5851102E-527.9997657
ACTACTG755.5851102E-527.99976515
CCTAGTA1154.8989023E-827.39107553
CACCCTA1154.8989023E-827.39107566
TAGTCGC657.956397E-426.92285241
TAGGGTT2400.026.2497822
ACTCCAG3450.025.36210623
TCCTAGT1251.09892426E-725.1997952
Produced by FastQC (version 0.11.5)