FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surE_T2_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surE_T2_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788581
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA96381.2221953103105452TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT34330.43533891889355697TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC16700.047.99344648
TCGTATG17500.045.7994646
AGTCACC18250.045.2597429
ACTACCC1550.045.1636246
TATCTCG16250.044.79947342
ACCGCTC18450.044.57942233
CACCGCT18400.044.5103532
CGTATGC18350.043.86869447
CGCTCAT18700.043.79627635
GTTACTA1600.043.752263
CAGTCAC19100.043.0623228
ACTCCAG19200.043.0203324
CCAGTCA19200.042.8380427
AGATCGG19650.042.4048041
CTCGTAT17450.042.3204245
CTCATTA19500.041.99950837
ATTATCT17900.041.84308240
CTCCAGT19850.041.7879225
CCGCTCA19750.041.64507734
GCCGTCT18100.041.57409752