Basic Statistics
Measure | Value |
---|---|
Filename | HLNTYAFXY_n01_surE_T2_R2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 788581 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA | 9638 | 1.2221953103105452 | TruSeq Adapter, Index 2 (97% over 37bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT | 3433 | 0.43533891889355697 | TruSeq Adapter, Index 2 (97% over 37bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGCC | 1670 | 0.0 | 47.993446 | 48 |
TCGTATG | 1750 | 0.0 | 45.79946 | 46 |
AGTCACC | 1825 | 0.0 | 45.25974 | 29 |
ACTACCC | 155 | 0.0 | 45.163624 | 6 |
TATCTCG | 1625 | 0.0 | 44.799473 | 42 |
ACCGCTC | 1845 | 0.0 | 44.579422 | 33 |
CACCGCT | 1840 | 0.0 | 44.51035 | 32 |
CGTATGC | 1835 | 0.0 | 43.868694 | 47 |
CGCTCAT | 1870 | 0.0 | 43.796276 | 35 |
GTTACTA | 160 | 0.0 | 43.75226 | 3 |
CAGTCAC | 1910 | 0.0 | 43.06232 | 28 |
ACTCCAG | 1920 | 0.0 | 43.02033 | 24 |
CCAGTCA | 1920 | 0.0 | 42.83804 | 27 |
AGATCGG | 1965 | 0.0 | 42.404804 | 1 |
CTCGTAT | 1745 | 0.0 | 42.32042 | 45 |
CTCATTA | 1950 | 0.0 | 41.999508 | 37 |
ATTATCT | 1790 | 0.0 | 41.843082 | 40 |
CTCCAGT | 1985 | 0.0 | 41.78792 | 25 |
CCGCTCA | 1975 | 0.0 | 41.645077 | 34 |
GCCGTCT | 1810 | 0.0 | 41.574097 | 52 |