FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surE_T2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surE_T2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692094
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA14240.20575239779567514TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT11900.17194196164104875TruSeq Adapter, Index 2 (97% over 37bp)
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC8980.12975116096946368No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG7180.1037431331582126No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG6960.10056437420350416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTG200.006951696752.4984370
ACTAGTA501.7100821E-434.99895537
CACGTAG400.002419125334.998959
GTCTAAC652.100133E-532.313731
TACTACC552.9914916E-431.8195275
CACTAGT450.00430791931.1101836
GTATGCC4200.027.50116348
CTACGTT906.1619485E-627.227311
TAGACAC808.667544E-526.24921462
AGTCACC4550.026.15495329
CTAGTAG700.001226275524.9992538
TATCTCG4100.024.75535842
GTGATAG2150.024.4196384
CCAGTCA5100.024.02062427
ACTCCAG5250.024.00101524
ACCGCTC5000.023.80100833
TCGTATG4900.023.57072346
CGGGGTT1052.0361627E-523.3376941
GTTACTA750.001831846723.334323
GCACACG5250.023.33263412
Produced by FastQC (version 0.11.5)