FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surD_T4_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surD_T4_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933151
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA21640.23190244665654328No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC21360.2289018604705991No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG21240.22761589496233728No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT19790.21207714507084063No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA17280.18517903318969814No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA16580.17767756772483767No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC15610.16728267986638817No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG14520.1556018264996769No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT13560.14531410243358256No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT12360.13245444735096465No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT12300.13181146459683374No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA12190.1306326628809271No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG11960.12816789565675865No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA10500.11252198197290685No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT10200.10930706820225237No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC10060.10780677510928027No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT9840.10544917167746698No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT9760.10459186133862579TruSeq Adapter, Index 3 (97% over 36bp)
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA9400.10073396481384042No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA9360.10030530964441982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT7150.043.564436
TGTACTC7200.043.264222
TCTAGTT8100.039.319187
CACTAAT5450.038.5344731
ACTCTAG8500.037.0571255
GTCACTA5700.036.2302929
CTAGTTG8850.035.196128
ACTAATG6150.034.14660632
GTTACTA2200.033.4075553
TCACTAA6400.032.8145130
CTAGTAG2250.032.6651738
AGTCACT6350.032.52167528
TAATGCG6350.032.51993634
TAGTTGT10050.032.038339
CACCCTA2300.031.95676866
GTTACCT10000.031.1485714
CCGAGTT450.0043056331.1146831
GACCCCT450.004309018631.10968215
AGACCCC450.004309018631.10968214
ACTAGTA2400.030.62523737
Produced by FastQC (version 0.11.5)