FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surD_T4_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surD_T4_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784979
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC32340.4119855435623119No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT20680.26344653806025387No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA20450.26051652337196285TruSeq Adapter, Index 3 (97% over 36bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA19960.2542743181664732No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT17660.22497417128356298No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA17570.22382764379684042No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT16310.2077762589827244No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA15670.1996231746326972No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA15630.19911360686082047No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG15610.19885882297488214No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC14930.19019617085297824No Hit
CCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTTCCTGACA14100.1796226395865367No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG13660.174017394095893No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT13510.17210651495135537TruSeq Adapter, Index 3 (97% over 36bp)
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCT13390.17057781163572527No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA13340.1699408519208794No Hit
GTTTTAACCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTT13000.16560952585992747No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA12870.1639534306013282No Hit
GATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCA12290.15656469790911604No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA11560.14726508607236627No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG11280.14369811166922938No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC10970.13974896143718493No Hit
GGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCG10930.13923939366530824No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG10910.13898460977936988No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA10460.13325197234575703No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA10050.12802890268402084No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA9730.12395236050900724No Hit
CGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGC9640.12280583302228466No Hit
TGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGC9630.1226784410793155No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA9290.11834711501836355No Hit
GTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTG9010.11478014061522665No Hit
CGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATC8910.11350622118553491No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTA8740.11134055815505892TruSeq Adapter, Index 3 (97% over 36bp)
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG8300.10573531266441522No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG8100.10318747380503174No Hit
ATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCG7970.10153137854643246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGGG305.8784167E-446.6664157
GTCACTA16550.042.2958430
CACTAAT16850.041.9582332
ACTAATG17000.041.38212633
TGCGCAT15900.040.7230538
AGATCGG17450.040.518121
ATGCGCA17400.040.0285237
TAATGCG17500.039.9997835
AATGCGC17850.039.80370336
TCACTAA17650.039.6598431
ACTCCAG17800.039.52225524
AGTCACT18000.038.69423329
TCCAGTC18400.038.2334926
CTCCAGT18400.038.04327425
CAGTCAC18500.037.8376328
CCAGTCA18550.037.54696327
CACACGT19600.036.0712313
TCTGAAC19700.036.06579219
ACGTCTG19900.035.879216
CTGAACT19850.035.79325520
Produced by FastQC (version 0.11.5)