FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surD_T3_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surD_T3_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128160
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT19280.17089774500070912TruSeq Adapter, Index 13 (97% over 38bp)
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT14270.12648915047510992No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC12840.11381364345482911No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA12800.11345908381789817No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC11650.10326549425613389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACTA452.6995622E-854.4547843
CTCTAGT1850.039.737286
TACTACC751.4895923E-632.6728745
AGGGGGG1305.456968E-1232.3138285
GTCTAAC703.480992E-530.0123521
CTAGTTG2450.029.999058
TCTAGTT2500.029.4003727
CACACGT7550.028.27725212
CTATAGG755.5891833E-527.99911511
GTATGCC6250.027.99911547
AGTCACA7550.027.81492228
ACTCCAG7600.027.63070323
GCACACG8000.026.68665511
GTTACCT2800.026.24916814
CCAGTCA8100.025.92510626
ACTATAG851.3092803E-424.70510110
CACTAGT851.3092803E-424.70510136
CTCCAGT8450.024.43709624
CACATTC8700.024.13823531
GTCACAT8600.024.0119329
Produced by FastQC (version 0.11.5)