FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surD_T3_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surD_T3_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807207
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA125651.5566019620741645TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT63480.7864153804414482TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGTCGTA20650.25582037816817743TruSeq Adapter, Index 13 (97% over 38bp)
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT12360.15312057501979046No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT12160.15064289581235046No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA11830.14655472512007453No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC11270.1396172233392426No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA11020.13652012432994262No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA10970.13590070452808264No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT9700.1201674415608388No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA9400.11645092274967883No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA8480.10505359839545493No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG8310.10294757106913095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG29250.049.552411
AGTCACA34750.042.40167629
GTCACAT34450.042.36454830
GTATGCC25550.042.33019348
ACTCCAG34550.042.14063324
CAGTCAC35150.041.81958428
CACATTC35450.041.56440732
CTCCAGT35350.041.38496825
TCCAGTC35450.041.3669526
CCAGTCA35750.041.31351527
CGTATGC25100.041.1368447
ACGTCTG36400.040.4796216
GAACTCC36800.040.03962722
TCACATT37100.039.90453331
TCTGAAC37150.039.85082619
TGCGTGA3250.039.84749661
ACATTCA37000.039.72860733
GCACACG37500.039.4788812
TGAACTC37350.039.45002421
CACACGT37400.039.39727813
Produced by FastQC (version 0.11.5)