FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surD_T3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surD_T3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753955
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC15020.19921613358887466No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA11930.1582322552406974TruSeq Adapter, Index 13 (97% over 38bp)
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA9310.12348217068657943No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT9060.12016632292378192No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA8870.11764627862405581No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT8480.1124735561140917TruSeq Adapter, Index 13 (97% over 38bp)
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA8230.10915770835129418No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA7700.10212811109416345No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA7680.10186284327313964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACTA751.4551915E-1146.6727263
TACTACC802.910383E-1143.755685
CTCTAGT2000.043.7556766
TTACTAC901.8189894E-1242.783334
ACTAGTA1051.4551915E-1136.6665637
CTCCTAG1102.3646862E-1135.00222451
CACTAGT1154.0017767E-1133.47816536
CTAGTAG1206.548362E-1132.08111638
ACTACCC1109.058567E-1031.8223156
AGTCACA5150.031.2641229
TCCTAGT1251.05501385E-1030.80195652
TACACTC1251.05501385E-1030.7978713
GTATGCC4200.030.00190448
CACCCTA1052.0247171E-829.99991266
GCCACTA1301.6734703E-1029.61530134
CCTATGC1301.6734703E-1029.61333745
TCTAGTT3100.028.2257277
TGTACTC3500.028.0054912
CCTAGTA1253.5561243E-927.9999253
CTAGTTG3150.027.7758568
Produced by FastQC (version 0.11.5)