FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surD_T2_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surD_T2_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615611
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG20070.12422544783366787TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACTA1400.035.0022133
TACTACC1450.033.795245
TCGGGGG5250.029.99911144
ACTCCGG10950.029.40734333
CACTAGT1551.8189894E-1229.35487736
GTCACTC11800.028.17887530
ACTCCAG11850.027.7637524
ACTAGTA1705.456968E-1226.7647437
CTAGTAG1705.456968E-1226.76391238
CCAGTCA12750.026.62831127
CACTCCG12150.026.50291332
CTAACGT2650.026.422491
ATCGGGG8200.026.03581443
TCACTCC12850.025.87632231
CTCTAGT2850.025.7911036
CTCCTAG2050.025.61058251
CACACGT13050.025.47817213
GCACACG12950.025.40465412
CAGTCAC13350.024.9071728
AGTCACT13500.024.63042429
Produced by FastQC (version 0.11.5)