FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surD_T2_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surD_T2_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771091
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG652.1035143E-532.30682445
GTCTAAC1202.2919266E-929.1753441
ACTCCGG2150.026.04581333
CTAACGT1701.4006218E-1024.7132321
ACTATTG1102.9224555E-522.2721344
TCGGGGG1102.9224555E-522.2721344
CTCTAGT800.00266376421.8786686
GGGGTAG800.002666774721.87441370
CACTCCG2750.020.3630932
ATCGGGG1901.35351E-820.26261143
ATCTAGT1055.4653076E-419.99946218
ACTCCAG3000.019.832824
TCTAACG1451.1141501E-519.3148372
GTCACTC2951.8189894E-1218.9825430
GTACTGC2254.623871E-918.66616433
AGGTGGT950.007201534718.424146
CTCCGGA3450.018.2603834
CGCGTCT1551.9781564E-518.069891
CCAGTCA3101.8189894E-1218.06403227
GCACACG3153.6379788E-1217.777312
Produced by FastQC (version 0.11.5)