FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_surD_T2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_surD_T2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532968
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG11010.2065790066195344TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT9140.17149247234355533TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAG200.00695062952.49880238
CTATAGG501.7075994E-435.005774
GAGTGGG400.002418543734.99920345
ACTCCGG6150.033.00737832
AGTCACT6500.031.76850728
CACTCCG6400.031.71802531
ACTCCAG6550.031.52599723
GTCACTC6650.031.05192629
TCCGGAG6850.030.145334
TCACTCC6750.030.07338730
CTAACGT1052.0134394E-830.0133951
CAGTCAC7000.029.99931727
TCGGGGG2800.029.99931543
CCAGTCA6900.029.92685326
CTCCGGA6800.029.85226233
ATCGGGG4700.029.78655442
TGAACTC7300.029.24590920
CTCGTGC500.00720775727.99936316
TCCAGTC7400.027.9047725
AGATCGG6150.027.898631
Produced by FastQC (version 0.11.5)