HLNTYAFXY_n01_PY79_T4_R3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HLNTYAFXY_n01_PY79_T4_R3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992496
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG	3134	0.3157695345875449	No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC	3028	0.30508939078847674	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA	2691	0.2711345939933259	TruSeq Adapter, Index 19 (97% over 38bp)
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA	2544	0.256323451177637	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT	2518	0.253703793264658	TruSeq Adapter, Index 19 (97% over 38bp)
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG	2369	0.23869113830181682	No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT	1977	0.1991947574599797	No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC	1969	0.19838870887137075	No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC	1928	0.19425770985475005	No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT	1869	0.18831310151375924	No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA	1868	0.18821234544018314	No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA	1842	0.18559268752720415	No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG	1833	0.1846858828650191	No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG	1830	0.18438361464429076	No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCG	1781	0.17944656703906112	No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA	1675	0.1687664232399929	No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT	1535	0.1546605729393368	No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT	1476	0.148715964598346	No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA	1428	0.14387967306669244	No Hit
GGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGC	1391	0.14015169834437619	No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC	1371	0.1381365768728539	No Hit
CCGCCAGCTGCTACAGCGCGGCGCAATTCAAAAAGGCGAGGTTTCTTATA	1334	0.13440860215053765	No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT	1244	0.12534055552868725	No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT	1183	0.11919443504054424	No Hit
GGCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGA	1180	0.1188921668198159	No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC	1159	0.11677628927471748	No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG	1147	0.1155672163918041	No Hit
CGGCCATTAATCTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAG	1143	0.11516419209749965	No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA	1127	0.1135520949202818	No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA	1104	0.11123470522803114	No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA	1084	0.10921958375650885	No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA	1073	0.10811126694717156	No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA	1011	0.10186439038545243	No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG	999	0.10065531750253906	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACTA	125	0.0	39.205334	3
CTAGTAG	135	0.0	36.29575	38
TACTACC	140	0.0	35.00476	5
TAGCTAG	40	0.0024194536	34.999474	66
ACTACCC	145	0.0	33.797703	6
CACTAGT	145	0.0	33.792595	36
TTACTAC	150	0.0	32.671112	4
ACTAGTA	165	0.0	31.817703	37
GAAGCTA	860	0.0	30.115824	37
TAGTAGC	165	0.0	29.696524	39
CTCTAGT	260	0.0	29.619415	6
AGCTATC	835	0.0	28.502563	39
CGGGGAG	160	1.8189894E-12	28.445671	1
CTATCTC	815	0.0	28.34313	41
AGTCTAC	125	3.5597623E-9	27.999578	11
CTCCAGT	925	0.0	27.999577	25
TCCTAGT	150	2.910383E-11	27.999577	52
AGTCACC	915	0.0	27.923075	29
CAGTCAC	930	0.0	27.849043	28
TATCTCG	830	0.0	27.830904	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content