FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_PY79_T4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_PY79_T4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906125
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC25070.27667264450269No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT22650.24996551248448062No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA20030.22105117947303077No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA19220.21211201545040695No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA19150.2113394951027728No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC18570.20493861222237547No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA17760.1959994481997517No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT17680.1951165678024555No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA16940.18694992412746586No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA16370.18065940129673058No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG14470.15969099186094632No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG14050.1550558697751414No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA12920.14258518416333288No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT12870.14203338391502277No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA12860.14192302386536074No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG12090.13342530004138503No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG11950.13188025934611672No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA11920.13154917919713066No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC11900.1313284590978066No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG11410.1259208166643675No Hit
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCT11280.1244861360187612No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA10390.11466409159884122No Hit
GGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCG10300.11367085115188302No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG9990.11024968961236031No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC9980.1101393295626983No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG9670.1067181680231756No Hit
TGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGC9370.10340736653331493No Hit
CACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAG9190.10142088563939854No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA9180.10131052558973652TruSeq Adapter, Index 19 (97% over 38bp)
ATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAA9080.1002069250931163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGC200.00695299452.4972712
GGGCCCA500.007205581328.003183
AGTCACC2600.026.92316229
GTGATCT3750.026.13341566
ACGTCTG2850.025.7881316
CTATCTC2500.025.20007941
TACGGAT6000.025.0931051
GTGGAGA2800.025.0097391
ATCTCTC3900.024.23084669
GTCTAAC750.001828584423.3424221
CACGTCT3200.022.96755415
TATCTCG2600.022.88468742
CACACGT3400.022.6458813
GTGATAG3800.022.1077734
CCCATGT4150.021.9277830
TCGTATG3050.021.80334946
ACTCCAG3300.021.21218924
GAAGCTA3500.020.99890937
ACTATAG850.003791296920.58716415
TGTGATC4800.020.4167365
Produced by FastQC (version 0.11.5)