HLNTYAFXY_n01_PY79_T3_R3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HLNTYAFXY_n01_PY79_T3_R3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	854364
Sequences flagged as poor quality	0
Sequence length	76
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT	5128	0.600212555772481	TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA	1654	0.19359429938527373	TruSeq Adapter, Index 7 (97% over 38bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	1477	0.17287713433618457	No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	1326	0.15520316867283734	No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC	1223	0.14314741726009053	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	1138	0.13319849619131893	No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT	914	0.10698016302185018	No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG	914	0.10698016302185018	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGTCACG	1065	44.036785	28
GTATGCC	1085	41.61217	47
CACACGT	1180	40.63488	12
CAGTCAC	1175	40.509926	27
GTCACGA	1165	40.2568	29
CTCTAGT	375	40.139675	6
GCACACG	1195	40.124817	11
TCACGAG	1170	39.785625	30
ACTCCAG	1170	39.785625	23
TCGTATG	1160	39.52517	45
CGAGATT	1190	39.11696	33
CTCGTAT	1075	39.069084	44
CCAGTCA	1225	38.285046	26
TCCAGTC	1215	38.024025	25
CGTATGC	1220	37.868187	46
AGCACAC	1280	37.46028	10
AGATTCC	1220	37.294426	35
CATCTCG	1125	36.71047	41
TCTGAAC	1320	36.32512	18
CTCCAGT	1285	36.225044	24