FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_PY79_T3_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_PY79_T3_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598129
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA112421.8795276604210795TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT36360.607895621178709TruSeq Adapter, Index 7 (97% over 38bp)
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC8780.1467910768412834No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT8110.1355894798613677No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA7970.1332488476566092No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT7350.12288319074982154No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC7140.11937224244268378No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTA7110.11887067839880694TruSeq Adapter, Index 7 (97% over 38bp)
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA6900.1153597300916692No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG6630.11084565369677778No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATGTCGTA6570.10984252560902413TruSeq Adapter, Index 7 (97% over 38bp)
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG6470.10817064546276807No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA6470.10817064546276807No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTA6470.10817064546276807TruSeq Adapter, Index 7 (97% over 38bp)
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT6470.10817064546276807No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA6430.10750189340426565No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA6240.1043253211263791No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA6130.1024862529654974No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA6020.10064718480461572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG21000.052.5246541
GCTAGCC200.00695130152.4983133
AGTCACG23000.049.30275729
CGAGATT22950.048.95266734
TCACGAG23200.048.87773531
GTCACGA23350.048.56374430
ACTCCAG23150.048.52975524
GTATGCC20950.048.4471348
TCGTATG21100.047.93684446
CTCCAGT23450.047.90890525
AGATTCC23500.047.6580436
CAGTCAC23700.047.5512128
CGTATGC21500.047.53334847
CCAGTCA23800.047.35141827
GAGATTC23850.046.95865635
TCCAGTC24000.046.95681826
GCACACG24150.046.9550112
CACACGT24100.046.907213
GATTCCA23200.046.7657337
CTCGTAT19550.046.72483445
Produced by FastQC (version 0.11.5)