FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_PY79_T3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_PY79_T3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617616
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA21740.3519986528846403TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT8180.13244475531721975TruSeq Adapter, Index 7 (97% over 38bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC7670.12418719722286986No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA6950.11252946814849356No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA6790.10993886168752104No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT6560.10621486489987306No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA6530.10572912618844071No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA6460.10459573586176524No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC6420.1039480842465221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG4950.038.18424629
GTCACGA5600.035.00222830
ACTCCAG5600.034.374424
CAGTCAC5550.034.0562228
TCACGAG5550.033.42555231
GTATGCC5400.033.0576648
CCAGTCA6000.032.08537327
CTCCAGT6000.032.08277525
GTGGAGA2200.031.8202061
CGAGATT5750.031.65162534
CGTATGC6000.029.74948347
TCTGAAC6850.029.63452119
AGATCGG6500.029.6172681
TCGTATG6050.029.50362246
GCCGTCT5800.028.9673652
GCACACG6450.028.75919212
CACGAGA6500.028.54027632
ACGTCTG6750.028.51802316
CACACGT6550.028.3201213
TCCAGTC6850.028.101726