Basic Statistics
Measure | Value |
---|---|
Filename | HLNTYAFXY_n01_PY79_T3_R1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 617616 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA | 2174 | 0.3519986528846403 | TruSeq Adapter, Index 7 (97% over 38bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT | 818 | 0.13244475531721975 | TruSeq Adapter, Index 7 (97% over 38bp) |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 767 | 0.12418719722286986 | No Hit |
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 695 | 0.11252946814849356 | No Hit |
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA | 679 | 0.10993886168752104 | No Hit |
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT | 656 | 0.10621486489987306 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 653 | 0.10572912618844071 | No Hit |
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA | 646 | 0.10459573586176524 | No Hit |
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC | 642 | 0.1039480842465221 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGTCACG | 495 | 0.0 | 38.184246 | 29 |
GTCACGA | 560 | 0.0 | 35.002228 | 30 |
ACTCCAG | 560 | 0.0 | 34.3744 | 24 |
CAGTCAC | 555 | 0.0 | 34.05622 | 28 |
TCACGAG | 555 | 0.0 | 33.425552 | 31 |
GTATGCC | 540 | 0.0 | 33.05766 | 48 |
CCAGTCA | 600 | 0.0 | 32.085373 | 27 |
CTCCAGT | 600 | 0.0 | 32.082775 | 25 |
GTGGAGA | 220 | 0.0 | 31.820206 | 1 |
CGAGATT | 575 | 0.0 | 31.651625 | 34 |
CGTATGC | 600 | 0.0 | 29.749483 | 47 |
TCTGAAC | 685 | 0.0 | 29.634521 | 19 |
AGATCGG | 650 | 0.0 | 29.617268 | 1 |
TCGTATG | 605 | 0.0 | 29.503622 | 46 |
GCCGTCT | 580 | 0.0 | 28.96736 | 52 |
GCACACG | 645 | 0.0 | 28.759192 | 12 |
CACGAGA | 650 | 0.0 | 28.540276 | 32 |
ACGTCTG | 675 | 0.0 | 28.518023 | 16 |
CACACGT | 655 | 0.0 | 28.32012 | 13 |
TCCAGTC | 685 | 0.0 | 28.1017 | 26 |