FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_PY79_T2_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_PY79_T2_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835334
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT48880.585155159493089TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA22520.2695927616977161TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA10900.043.6668536
ACTCGAT10800.043.09901437
GTATGCC11250.042.62196747
ACATTAC12100.041.0765932
ACTCCAG11800.040.93195723
TCGATCT9600.040.8330939
TCGTATG11900.040.58799445
AGTCACA12000.040.2521728
GCTAGCG350.001255608539.9997626
ATTACTC12150.039.7528534
CATTACT12450.039.3595533
CCAGTCA12450.038.79495226
TTACTCG12500.038.3597735
GTCACAT12700.038.3091529
TCCAGTC13000.038.2305425
CTCCAGT12750.038.15663524
CACACGT12900.037.71069712
CAGTCAC12850.037.58957327
CGTATGC12850.037.58732246
GCACACG13000.037.42061211
Produced by FastQC (version 0.11.5)