FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_PY79_T2_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_PY79_T2_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829646
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA13460.16223787012774124TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA4050.030.24631537
ACTCGAT3750.029.86607438
ATTACTC4450.028.31404735
TTACTCG4550.027.69176136
GTATGCC4300.027.67387248
AGTCACA4550.026.92254429
ACATTAC4750.026.5257933
CTCTAGT1358.067218E-925.9301016
CTAGTTG1508.8220986E-1025.6661598
GCCCAAC700.001226408824.99950455
CACACGT5050.024.95000313
ACTCCAG4800.024.79117424
AGATCGG5250.023.3441281
AGGGGGG750.001830886723.3370915
GCACACG5300.023.1127512
CAGTCAC5150.023.10633928
TAGGGTT2155.456968E-1222.79024722
CATTACT5650.022.30044434
CCAGTCA5350.022.24255227
CTAGGGC952.7808754E-422.10482664
Produced by FastQC (version 0.11.5)