FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_PY79_T2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_PY79_T2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995390
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA16550.1662664885120405TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACTC4650.027.84856235
TACTCGA4350.027.35543837
AGTCACA4800.026.25047129
GTATGCC4750.025.05308248
GTGCCAC851.309135E-424.70508636
ACATTAC5250.024.66586933
TTACTCG5250.024.66586936
ACTCGAT4300.024.41781638
CACACGT5650.024.1585113
ACTCCAG5400.023.33375424
GCACACG5650.022.91961312
CAGTCAC5900.021.94954528
GTCCTAG800.002667398221.87429411
TAGGGTT2600.021.53884922
CATTACT6050.021.40426634
GATTCGA3650.021.0962684
AGGTGGT1356.0223865E-620.7411146
CCAGTCA6100.020.65610927
TCGTATG6350.019.84287546
CTAGTGG1601.1858792E-619.68785369
Produced by FastQC (version 0.11.5)