FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_6_ctrl.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_6_ctrl.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35910299
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA2916130.8120595152939272No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA1334820.37170951987896284No Hit
GAAGATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGA1040590.2897748080571538No Hit
TTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAG1040370.289713544295468No Hit
CTCAACTTTACCGGATTTCAAAGCTCGAGCAGATACATATACTTTTTTAG1023750.28508534557175363No Hit
ATTTTTTGTTAAAGCGATCAACACGACCATCAGCAGAAGCGAATTTTTGA924080.257330076811669No Hit
CTAACGTTCTGTAAGCCATGTTTTGTTCCATCGTACTGCAAACGGCTCAT885050.24646132854532898No Hit
TGAAGATTGACTTTTCGTCTTGATGATACTTTGGGCAAGGATAGTATATA866110.24118707560747404No Hit
ATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGAAACA715060.19912393377732665No Hit
CTCGATTACAATACCCATACTGCGGGCAGTACCTTCAACCATGCGCATTG622130.17324556389797813No Hit
CGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAAA614130.17101779074576906No Hit
CTCCGAAATAATAACTCTGTTTCCACACGGGATTCAGGGCGGAAGGAGAT590950.1645628180372433No Hit
GTCCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCT556960.15509756685679504No Hit
CCGGCAACTGCGTCTTTAAGCGCTTTACCTGGTTTGAAAGCAGGTACTTT521880.1453287815843583No Hit
CTTGAATCTGTAGAGGAAAGTCCATGCTCGCACGGTGCTGAGATGCCCGT491350.13682704229224046No Hit
TGCAGATCTTGAATCTGTAGAGGAAAGTCCATGCTCGCACGGTGCTGAGA477440.13295350172383694No Hit
CTAGTTTGTTAATTAACTCACGGTAACGAGTTACGTCTTTATTACGCAGA472590.1316029142503102No Hit
CGAAATAATAACTCTGTTTCCACACGGGATTCAGGGCGGAAGGAGATGGA454550.12657928579207875No Hit
GTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAGCAAGAATAGTGA454410.1265402997619151No Hit
GTCCGTTCACTTTTTTCGCTAGTCTTGATTTGTATCGAGCTGCGGTATTT430250.11981242484224372No Hit
CTATCTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTT414150.11532903137342297No Hit
CGCTTCTTTAGTAACGTTAGCAGCTTGTGGTCCGCGGTTTCCTTCAACGA409030.11390325655600919No Hit
AAAGCATCTAAGATTATATGAATGTTAAAAAGTAGATGTTTTGTGCAAGT398410.11094588769645164No Hit
TAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAGCA381660.10628148765901392No Hit
GTGAATTTAAGCGTTATGATCGCTTTAGGAGGAAATTTCATGTTAGAAGG378120.10529569803916142No Hit
TAACGTTCTGTAAGCCATGTTTTGTTCCATCGTACTGCAAACGGCTCATC371300.10339652142690317No Hit
CCGGATTTCAAAGCTCGAGCAGATACATATACTTTTTTAGGTTTTCCGTT365660.1018259413545958No Hit
AAAGGATAGTATGAAGATTGACTTTTCGTCTTGATGATACTTTGGGCAAG361740.10073433251001336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT202000.034.5920371
GGGTGGC233400.032.9602966
GGTGGCA233400.032.93029867
TGGGTGG248200.031.06522265
GTCTAAC54200.029.9699251
CTCCGAA163800.029.4938961
CTATCTT106050.028.8514541
AGATTTA273050.028.8429833
AGTGTAG271950.028.58356556
GTGGCAT274500.028.08896368
AGAAAGT809950.027.7760039
GAACTCC53700.027.3094921
TAAGAGT833400.026.88660820
CTCAACT306750.026.2944981
TGTAAGA862450.026.14287618
GAGGCAA244100.026.13937270
TTGTAAG878150.025.71846417
ACACGAC296350.025.65244321
GACGGCC298850.025.64919749
CGACCAT294800.025.59735724
Produced by FastQC (version 0.11.5)