FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_6_Fe500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_6_Fe500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19721193
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA316850.1606647224637982No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA198590.10069877618458477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT86600.040.5054971
AGGCCTA96250.037.56385422
GGCCTAA106900.034.18167523
TCGATCG51900.033.922493
CAGGCCT109800.032.89636621
CGATCGA56500.031.6562524
GCCTAAC116400.031.42200324
TCAGATT116150.031.0995562
TTAGTAT57050.030.91956311
CAGATTG118600.030.751773
GTCGAAC88900.030.70798339
CGATTAG57900.030.5865448
CGGCAGG119550.030.35962918
TGAACGC123850.029.7571168
AGATTGA135500.029.344464
ACACATG137400.026.28842729
GGCGGCA139900.026.16811416
CTCGATC67650.025.869972
TTGAACG150150.025.4546287
GATCGAT71100.025.4511875
Produced by FastQC (version 0.11.5)