FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_5_ctrl.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_5_ctrl.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26812181
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA2043380.7621088340407668No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA790260.29473917097605745No Hit
CTCAACTTTACCGGATTTCAAAGCTCGAGCAGATACATATACTTTTTTAG744380.27762754548016816No Hit
ATTTTTTGTTAAAGCGATCAACACGACCATCAGCAGAAGCGAATTTTTGA634910.23679908769823685No Hit
CTAACGTTCTGTAAGCCATGTTTTGTTCCATCGTACTGCAAACGGCTCAT619070.23089132510331778No Hit
TTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAG618030.2305034417006211No Hit
TGAAGATTGACTTTTCGTCTTGATGATACTTTGGGCAAGGATAGTATATA583020.2174459436925329No Hit
GAAGATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGA571020.21297036596910934No Hit
CTCCGAAATAATAACTCTGTTTCCACACGGGATTCAGGGCGGAAGGAGAT480360.17915737626864447No Hit
ATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGAAACA417740.15580231984857926No Hit
CTCGATTACAATACCCATACTGCGGGCAGTACCTTCAACCATGCGCATTG417680.15577994195996214No Hit
CTAGTTTGTTAATTAACTCACGGTAACGAGTTACGTCTTTATTACGCAGA410890.153247510898125No Hit
CCGGCAACTGCGTCTTTAAGCACTTTACCTGGTTTGAAAGCAGGTACTTT381630.14233456054917726No Hit
CGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAAA362550.13521839196893382No Hit
GTCCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCT357710.13341324228715298No Hit
CTTGAATCTGTAGAGGAAAGTCCATGCTCGCACGGTGCTGAGATGCCCGT340680.1270616515679944No Hit
CTATCTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTT330530.12327605874359866No Hit
CGAAATAATAACTCTGTTTCCACACGGGATTCAGGGCGGAAGGAGATGGA310930.11596594846200689No Hit
TGCAGATCTTGAATCTGTAGAGGAAAGTCCATGCTCGCACGGTGCTGAGA293170.10934209343134005No Hit
AAAGCATCTAAGATTATATGAATGTTAAAAAGTAGATGTTTTGTGCAAGT286160.1067276101112401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGC149900.032.734966
GGTGGCA150800.032.4003467
CTAACGT147850.032.3689421
GTTGGGG25600.032.12841836
TGGGTGG161250.030.53912765
CTCCGAA125450.029.162651
TAAGAGT530700.028.7084120
ACGAACC197050.027.9752870
CTATCTT85950.027.5755441
AGTGTAG180750.027.43783256
TGTAAGA560950.027.3347218
GTGGCAT184000.026.68729468
GAGGCAA156950.026.62645570
AGAAAGT580050.026.4158929
TTGTAAG585000.026.2701517
CTCAACT223000.026.21761
GGAGGCA159250.026.19794369
TTAGTGG35600.025.9569383
AGAGTTT600050.025.65294822
CACTAGG163200.025.62781764
Produced by FastQC (version 0.11.5)