FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_5_Fe500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_5_Fe500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18969958
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA219870.11590431565531142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAGT76050.020.84769455
AATAGTG76850.020.72175656
GGGTGGC19100.019.24079366
GTAAGAG88750.018.06183619
GGTGGCA21250.017.95290267
CTAACGT62700.017.6990661
TAAGCCA58850.017.54419512
TGGGTGG22400.017.34357565
TAAGAGT93750.017.02387220
ACACTAG24750.016.5453363
GCCATGT63200.016.1705115
GTAAGCC66200.015.70204911
AGAATAG106300.015.04708354
TACTCAG76950.014.50912158
ACTCAGG72350.014.22223659
TAGTGAA114700.014.18889658
ACCTCGT74200.013.58519752
GTGTGGT29150.013.56779453
TACTGCG40050.013.37068618
CTCTCGC33650.013.31352733
Produced by FastQC (version 0.11.5)