FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_4_ctrl.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_4_ctrl.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25963222
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA1276150.4915222001337123No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA522420.2012153961476738No Hit
TTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGAG459100.1768270517426535No Hit
CTCAACTTTACCGGATTTCAAAGCTCGAGCAGATACATATACTTTTTTAG427600.16469450517351045No Hit
TGAAGATTGACTTTTCGTCTTGATGATACTTTGGGCAAGGATAGTATATA381680.14700794839715967No Hit
GAAGATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGA370550.14272111527606243No Hit
ATTTTTTGTTAAAGCGATCAACACGACCATCAGCAGAAGCGAATTTTTGA357880.1378411354338071No Hit
CTAACGTTCTGTAAGCCATGTTTTGTTCCATCGTACTGCAAACGGCTCAT315420.12148723297901932No Hit
ATTTAACCTTAAGACACTGTGCTACAGGTTGTATCTTAGGAGGTGAAACA274550.10574573525581686No Hit
CTCGATTACAATACCCATACTGCGGGCAGTACCTTCAACCATGCGCATTG265250.10216374531635557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC118900.034.73520721
ACTCCGC126600.032.37380632
GGGTGGC91050.030.40613766
CAGTCAC136500.030.33362427
GGTGGCA93900.029.48326567
TGGGTGG97200.028.37399565
CACTCCG146300.028.3256331
GTCACTC145750.028.3124529
CCAGTCA147700.028.07992226
CACGTCT153100.027.34081814
ACACGTC155800.027.13657213
TCCAGTC161400.025.78317325
AGTCACT164250.025.72018628
CTCCAGT160750.025.69216724
AGCACAC167100.025.38526310
CACACGT169750.025.0920612
TAAGAGT375400.024.90286620
TCACTCC166250.024.82129130
CTCCGCG167950.024.67415233
TGAACTC168850.024.66695820