FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n01_4_Fe500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n01_4_Fe500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25184638
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA414730.16467578370592423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGC40350.023.5067366
AATAGTG146050.021.66330156
GAATAGT146000.021.59867755
GGTGGCA45300.021.01537367
TGGGTGG46000.020.84754265
TAAGAGT161200.020.08375420
GTAAGAG166800.019.34633619
ACACTAG47500.018.56820363
TGTGCTA50500.017.67327122
CTAACGT84150.017.2656121
AGAATAG185050.017.23044854
AGTGTAG60000.015.98295556
TAGTGAA199500.015.96453758
CTCAACT82150.015.7255841
CACTAGG57700.015.64973164
AGTGAAT210750.015.49429659
ACACGAC63550.015.42093221
GTGTGGT58400.015.28250853
AGAGTTT220300.015.09305822
GCCATGT89350.015.04149515
Produced by FastQC (version 0.11.5)