FastQCFastQC Report
Thu 10 May 2018
HLNNTBGX5_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNTBGX5_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300864884
Sequences flagged as poor quality0
Sequence length98
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17869540.5939390387613331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8946750.066.7136151
AGCAGTG10610800.060.8170932
CAGTGGT14663050.054.4849974
AGTGGTA13849950.054.2576375
GCAGTGG14978150.053.003243
GGGCAGT2524900.048.2636531
GTGGTAT16827200.045.3718836
TGGTATC17027650.045.0123567
GGTATCA15949050.044.7797628
GTATCAA15870050.043.4908879
GGCAGTG3889100.035.7652632
GGTATCG2017700.027.3264668
CAACGCA15106700.024.76125112-13
ATCAACG15190850.024.30233210-11
TACATGG19259200.024.09434122-23
AGTGGTC2058600.024.0664225
AGAGTAC20036100.024.00499518-19
AGTACAT20042350.023.89335420-21
ACGCAGA16399150.023.82991614-15
GCAGAGT19389350.023.66868816-17
Produced by FastQC (version 0.11.2)