FastQCFastQC Report
Thu 10 May 2018
HLNNTBGX5_n02_10xgenomicscarlosbrunofirsttrial043018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNTBGX5_n02_10xgenomicscarlosbrunofirsttrial043018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11712230
Sequences flagged as poor quality0
Sequence length98
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118850.058.415628
AGTGGTA112800.055.386235
GTATCAA148200.055.1052259
TACCCTA82900.050.7819339
GTGGTAT151000.049.450146
TCGGCGC101600.049.1782657
TTTACCC84450.048.432767
TTACCCT86350.048.059938
TGTCGGC128950.047.9857255
AAGTGGT124750.047.7543074
GGTGTCG168250.047.737893
GAAGTGG154350.044.3774223
GTGGTGT352200.043.4644931
TGGTATC161200.042.783247
GTGTCGG158700.041.161834
GTGAAGA364750.039.3963851
TGGTGTC307500.039.3870852
GTGAAGG233850.039.1432041
GGGATGG113350.037.6588551
GTGATGT238350.037.4006581
Produced by FastQC (version 0.11.2)