FastQCFastQC Report
Thu 10 May 2018
HLNNTBGX5_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNTBGX5_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300864884
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTTCATCTTTCCCTCGCGGTACT13135920.4366052902338712No Hit
TCCAGCACCGGGCAGGTGTCAGACTC5998720.19938252414994367No Hit
TTTCATCTTTCCCTCGCGGTACTTGT4887500.16244833677565376No Hit
TTTTCATCTTTCCCTCGCGGTACTTG4680710.15557515180136475No Hit
CCTTCCCCTAAATGATAAGGTTCAAT3821220.12700784316191585No Hit
CTGGTCTGCATCGTTTATGGTTGAGA3044750.10119991271563616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT887350.017.9946671
GACTGCG1207700.017.8865531
GGCTCGA1124000.017.8645691
TGACTAG1127050.017.8641621
AGGACGA531000.017.7635788
TCCACGA1279500.017.7496688
CGAGCGA488000.017.6057056
TACGCCG1078650.017.49958810
GGCGACT1118450.017.4549391
ATAGACC877950.017.4385971
GCGTATG1204800.017.4314272
GTCGAAC963550.017.3919778
CGGAGCT1013050.017.3737161
GATCTAG1364800.017.3532451
ATCGAGT962100.017.2912651
CGGACTG915500.017.231531
ATGGCCA945150.017.2028063
TACGGGC892300.017.1525421
CGTATGT1224900.017.1409973
GGGAGAT666850.017.1059761
Produced by FastQC (version 0.11.2)