FastQCFastQC Report
Thu 10 May 2018
HLNNTBGX5_n01_10xgenomicscarlosbrunofirsttrial043018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNTBGX5_n01_10xgenomicscarlosbrunofirsttrial043018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11712230
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGA44700.017.996271
GCGTATG52900.017.8256422
GCGCGAT35900.017.7532941
GACTGCG48100.017.7434431
CGGACTG34200.017.7288461
TACGCCG46450.017.67425210
TCCACGA56900.017.6618238
ATAGACC34450.017.6582781
TGACTAG46700.017.6111981
CGTATGT53750.017.5993353
AGGACGA25600.017.5773878
CTGATAG50300.017.5641331
GAAGACC25600.017.53846
GTCGAAC39800.017.5118268
CTAATGG43650.017.4664521
ATGGCCA41050.017.4658433
AGACCGG30050.017.4035958
CGAGCGA20550.017.3716056
GATGGCC41500.017.3248672
AAGACCG24200.017.3133957
Produced by FastQC (version 0.11.2)