FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bw-28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bw-28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11982162
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG275780.23015879771947667No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT271510.22659516704915192No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG213560.17823160795188714No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG210080.17532729068426883No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG206680.17248973933084866No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT182090.15196756645420084No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC173020.14439798093198875No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC159690.13327311047872664No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT157680.1315956168844988No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG156790.13085284608904468No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAGGCCCCGCTTCCGACTCACGGAATAA137910.11509609033828787No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGCCGACCCTGATCT126460.10554021886868163No Hit
GTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTG124340.10377092214243139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA37000.042.063881
CTGACAT52350.037.969981
ACATGCG61100.032.3451164
CATGCGT61700.032.0305755
CTACACG4650.031.89413312
TTCGATC22650.030.7612382
ATGCGTG64800.030.5514746
CTCGTAT6950.029.79134444
GATCCTG24950.029.7209845
TCGTATG7350.029.57854845
TGCGTGC67300.029.4678217
GTCACGT7300.029.30304329
ACATCCG56850.027.7975255
GGTGGAT26450.027.7746873
CTGGTCG59100.026.73667569
CGCTATG86800.026.35346429
TAGTCAT59450.026.1649536
GCTATGA87250.026.14096530
GGCAACG15850.026.1139552
TATGCCG7800.026.10233148
Produced by FastQC (version 0.11.2)