FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bw-25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bw-25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9227150
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG182430.19771001880320577No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG142400.15432717578017047No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT133500.14468172729390982No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT122990.13329142801406718No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG122570.13283624954617623No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG121870.13207761876635798No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT117780.1276450474957056No Hit
GGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGAC105760.11461827324796932No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC105340.11416309478007837No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA104540.11329608817457178No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG99170.10747630633510889No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGCCGACCCTGATCT97400.10555805422042558No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAGGCCCCGCTTCCGACTCACGGAATAA93940.10180825065160964No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC92460.10020428843142248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA24600.042.224721
CTGACAT39300.036.6166761
ACATGCG41500.033.741824
CATGCGT42150.033.1396565
ATGCGTG42900.032.4798976
CGTGAAC17650.030.88676364
CTCGTAT5950.030.74132344
TGCGTGC46850.029.5942277
GTGCGAG50650.027.37393210
AGTCACG7100.026.72584528
CGGGACT27350.026.6232171
GTGAACC21250.025.97821665
TGCGAGT53850.025.87534911
TAGTCAT41950.024.9115776
TTCGATC15800.024.8867932
GGCAACG14800.024.2377782
GTCGAAT5850.024.185961
GCAACGG14850.023.6903423
GAGTCGA59450.023.43797514
AGTCGAC59750.023.32143215
Produced by FastQC (version 0.11.2)