FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bw-22_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bw-22_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820120
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT34710.12307986894174716No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG33910.12024311022226006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA8950.031.6145941
ACATGCG9100.029.5604654
ATGCGTG9550.028.8898096
CGGGACT6450.026.2139911
CTGACAT10850.026.07841
CATGCGT10500.025.9475175
TGCGTGC12550.022.5334747
TGCGAGT11600.022.29776211
ACATCCG8450.022.05128155
TTACGGG2301.4551915E-1120.9922148
GTGCGAG12900.020.8527310
ACCTAGG7100.020.40088310
GTCCCTA9250.020.1330728
AGTCGAC12850.020.12907615
CCTATTA7900.020.08115811
GAGTCGA12600.019.98103714
CTATAAG9200.019.88137646
CTACCCG8200.019.767066
TAGGTAC7400.019.57416713
CGCTATG13050.019.29956629
Produced by FastQC (version 0.11.2)