FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bw-22_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bw-22_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3451252
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG54790.15875398261268664No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT42030.12178189248423471No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG40010.11592894404697195No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG39820.11537841919396208No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT38270.11088729539309214No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT36440.10558487180883921No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG36370.10538204686299348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA7100.039.856831
ATGCGTG14650.029.6652016
CGGGACT9300.028.9440251
CTGACAT15600.028.3160481
ACATGCG15200.027.6841144
CATGCGT16150.026.4827795
ACATCCG12600.025.19462655
TGCGTGC17150.024.7374767
TTCGATC6000.024.7201162
CTATAAG13750.023.59104746
CTACCCG11000.023.2034976
TATAAGC14200.022.84344347
TGCGAGT18300.022.80597711
GAGTCGA18950.022.38774314
TAGTCAT13000.022.0216146
TAGACCG4900.021.82917658
CGGAGTC14650.021.66752639
TAAGCAA15100.021.4784949
GTGCGAG19550.021.34779510
CCCTGGT14550.021.34124667
Produced by FastQC (version 0.11.2)