FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bs-28_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bs-28_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9654791
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG189550.1963273984905525No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT184170.19075503550517045No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG149420.1547625422445706No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT143570.1487033743143689No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG143050.14816478160946206No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG132800.1375482907915873No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC127710.13227629681471095No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT125910.13041193745157198No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC108660.11254516022149003No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG107750.11160262298790309No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCTACCATGGTGGTGACGGGTGACGGAGAATTAGG99130.10267441314887087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA27950.041.3602141
CTCGTAT9350.035.06269542
CTGACAT41200.033.9217031
TCGTATG9600.033.79065743
CGTGAAC15150.030.97314564
ACATGCG46450.029.7760414
CATGCGT46100.029.7028355
ACGTCTG12000.029.60617615
ATGCGTG46400.029.4364556
TATGCCG11100.029.22480846
CACGTCT12400.028.37194314
TGCGTGC49100.028.0987387
CATTTTA12050.028.06052634
ATGCCGT12600.026.56719847
CAGTCAC12500.026.49473227
GTGAACC17650.026.1947765
GGTGGAT25000.026.0754833
CGGGACT35050.025.499491
GCAACGG14750.025.4885463
ACATCCG42100.025.48849155
Produced by FastQC (version 0.11.2)